Project

Part:BBa_K258011:Design

Designed by: Cihan Tastan and Ozkan IS   Group: iGEM09_METU-Gene   (2009-10-10)


Oxygen-dependent EGF synthesizing with GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1892
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3339


Design Notes

design with biobrick assembly


Source

EGF will be activated with oxygen presence using tetR inverter mechanism. Therefore,with the absence of oxygen tetR will be synthesized so that EGF and GFP synthesize will be stopped.

References